Protein X has a 100 nM affinity for ligand Q. How much of ligand Q is required for the following fractions of protein X to exist in the bound state? c) 0.50 d) 0.90

Protein Z also binds ligand Q. At a concentration 200 mM Q, of protein Z is 70% bound. Which protein (X or Z) has a greater affinity for ligand Q?

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