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Q3 (15 pts). Maleic acid (represent as H2M) buffers are commonly used in nucleic acid extraction. Describe the preparation of 100.0 mL pH 6.5 buffer

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Q3 (15 pts). Maleic acid (represent as H2M) buffers are commonly used in nucleic acid extraction. Describe the preparation of 100.0 mL pH 6.5 buffer solution using 100.0 mL of 0.100 M sodium maleate (represent as Na2M) solution and solid sodium hydrogen maleate (represent as NaHM). Find mass of NaHM to prepare the buffer solution. Assume that addition of solid NaHM does not change the total volume. Hint: A sketch of buffer pairs at different pH values may be helpful. First, decide on the buffer components in the final buffer solution. Show how you have decided For H2M, K.,: 1.3 x 102 K2: 5.9 x 10-7 MW of NaHM: 139.06 g/mol

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