Q.3 Consider the growth of a microorganism in batch culture, inoculated at a density of 0.1 g/L, growing on glucose as the limiting substrate with initial concentration S0 = 10 g/L. After a lag time of approximately 4 hours, the culture grows exponentially, with a doubling time of 2 hours. Deceleration phase is reached after a total time of 15 hours when the substrate is consumed completely. Write your assumption clearly. Estimate (i). Maximum growth rate (ii). The total time in culture to reach deceleration phase if so were 2 g/L, assuming that this concentration is also sufficient to support maximal growth. [15 marks]