Question 1 (a) A peak with a retention time of 407s shows a poorly resolved second peak which appeared 10s after it. The first peak had a width of 16s. The calculated resolution between the first and second peak was 0.74. Separation took place on a 30cm column. 1. What is the width of the second peak? II. (3 marks) What is the average number of theoretical plates for resolution of 0.74? (5 marks) What is the plate height for resolution of 0.74? (2 marks) What is the average number of theoretical plates to achieve good resolution at the same retention times? (6 marks) What is the column length to achieve good resolution at the same retention times, assuming no change in plate height? ( 6 marks) IV. V. (b) 2.60g of plant tissue sample was analysed and found to contain 3.6ug of Zinc. What is the concentration of Zinc in the plant in ppm