Question 1 The nitrogen requirement for living cells is provided from protein metabolism. However, when commercial processes are used for cell growth, ammonium sulphate, (NH4)2SO4 is typically used as the source of nitrogen. Determine the amount (in kg ) of ammonium sulphate required for a process in which the final cell concentration is 42g/L in a 600L volume of process mixture. Assume that the cells consist of 9%N on mass basis and that ammonium sulphate is the only nitrogen source