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Question 1: You are in charge of designing a steady-state CHEMSTAT bioreactor. Your design requirements are a) the CHEMSTAT needs to consume 90% of initial

Question 1: You are in charge of designing a steady-state CHEMSTAT bioreactor. Your design requirements are a) the CHEMSTAT needs to consume 90% of initial substrate concentration from the feeding stream. b) It needs to produce 5g/L of the cells at the outlet. c) The flow rate of the pipe needs to at 2L/hour. You can assume a perfect mixing in the reactor, no cell death occurs, no cell feed at the inlet, and Monod growth kinetics. What is the size of the reactor you need? The other constants for the cells are: = 0.8 / = 0.2/, _/ = 0.7

Question 2: Derive expressions for substrate concentration, cell concentration, and productivity of a CHEMSTAT for the Blackman model when S<2Ks, in term of model constant (max and Ks), dilution rate (D), yield constant (Yx/s) and initial substrate concentration (S0). Assume: 1. Feed stream contain no cells 2. Minimal cell death 3. Steady state. Blackman equation: = 2 Question 5: Derive the expression of optimal dilution rate () of ideal chemostat for productivity in terms of the constant, /, ,, 0. Assume standard operating condition and the specific growth rate model for the cells is = .,

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