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QUESTION 3 . Calculating oxygen transport properties in a bioreactor. ( 1 5 pts ) We have a 1 0 - liter ( broth volume
QUESTION Calculating oxygen transport properties in a bioreactor. pts
We have a liter broth volume fermenter that is well mixed and is supporting the growth of the yeast Pichia pastoris at using methanol as the carbon and energy source. The agitator is running at and we are sparging in air at a rate of SLPM standard liters per minute The head pressure on the fermenter is atm atm absolute pressure The exit oxygen concentration is being monitored by a mass spectrometer, which tells us that the exit gas has an oxygen concentration of The dissolved oxygen probe was calibrated to with oxygen saturated medium at and with atm head pressure and now reads
a What is the oxygen uptake rate mmolesLmin
b What is the overall oxygen transfer coefficient, mmolesLmin atm
c If we know from previous work with this vessel and fermentation that is proportional to what will be the new dissolved oxygen reading if we increase the rpm to
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