Question 5 The following Hill graphs are for the binding of Ligands to protein X and protein Y. L+Px = Px. L+ Py = PyL 5 5 Slope = 2.2 4 4 3 3 Log f/1-1 2 2 1 1 0 0 -1 -1 Slope = 0.6 -2 -2 -5 -4 -3 -2 -1 0 1 2 -5 -4 -3 -2 -1 0 1 2 Log [L] Log [L] A) Comment on the shape of the binding curves. Also comment on the binding cooperativity based on the figures above. B) How many ligand binding sites do you think each protein will have? Explain your answer. C) Explain weather protein X or protein Y will bind its ligand more tightly at 50% fractional occupancy. D) Compare the affinities of ligand binding for both proteins at in at high and low ligand concentrations. E) Sketch the ligand binding isotherms for proteins X and Y. In the Y-axis you should have fractional saturation, and ligand concentration in the X axis