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Question: A pharmaceutical chemist is tasked with identifying the presence of a specific drug compound, 'Drug A', in a biological fluid sample. The analysis involves
Question: A pharmaceutical chemist is tasked with identifying the presence of a specific drug compound, 'Drug A', in a biological fluid sample. The analysis involves a sequence of sophisticated spectroscopic techniques. The sequence of the analysis is as follows: The sample is first subjected to high-performance liquid chromatography (HPLC) to separate 'Drug A' from the biological matrix. The fraction containing 'Drug A' is then analyzed using mass spectrometry (MS) to determine its molecular weight. To further confirm the structure of 'Drug A', nuclear magnetic resonance (NMR) spectroscopy is performed. Finally, to quantify 'Drug A', the chemist uses ultraviolet-visible (UV-Vis) spectroscopy at a specific wavelength. Based on this sequence, what is the most critical factor or consideration for ensuring the accurate identification and quantification of 'Drug A' in the sample? Options: A. The choice of solvent for HPLC B. The resolution of the mass spectrometer C. The purity of the NMR solvent D. The calibration of the UV-Vis spectrometer Hints: Consider the role of each analytical technique in the identification and quantification process. Reflect on the aspects that are critical for the accuracy and specificity of each technique. Think about the challenges in analyzing complex biological matrices. Don't use chatgpt otherwise I will give you disupvote
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