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These results were obtained: Y = 12.5 cm Xa=10.0 cm WA= 1.0 cm Xa=8.5 cm WB=0.9 cm Calculate the Retardation Factor (Re) factor for
These results were obtained: Y = 12.5 cm Xa=10.0 cm WA= 1.0 cm Xa=8.5 cm WB=0.9 cm Calculate the Retardation Factor (Re) factor for both A and B 2 points Retardation factor (R): The ratio of the distance traveled by the center of the spot to the distance simultaneously trav- eled by the mobile phase and is used in planar chromatography. Using the symbols in Figure 3: R = bla Calculate the Chromatographic Resolution Factor(R) between A and B. Resolution (R): The resolution is the separation of two components in a mixture, calculated by: R = 2 x (tez-t)/(W + W) 2 points where t, and t are the retention times of the two components; and W, and W, are the corresponding widths at the bases of the peaks obtained by extrapolating the relatively straight sides of the peaks to the baseline. Where electronic integrators are used, it may be convenient to determine the resolution, by the equation: R = 1.18 x (tz-ta)/(W/2 +WN2)
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