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(b) A mixture of proteins is separated on a column with a stationary phase of carboxymethylated cellulose. The internal diameter of the column is

 

(b) A mixture of proteins is separated on a column with a stationary phase of carboxymethylated cellulose. The internal diameter of the column is 0.75 cm and its length is 20cm. the dead volume is 3 ml. the flow rate of the mobile phase is 1ml/min. the pH of the mobile phase is adjusted to 4.8. Three peaks appear upon the chromatogram corresponding to the elution volumes V, V and V3 at 12 mL, 18mL and 34mL respectively Does this arise from an anionic cationic phase? Give reasons for your answer [2 marks]

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