Question: What is the concentration of amino acid in an unknown solution?

Your task is to design and conduct an experiment to quantify the amino acid concentration in

an unknown solution using Ninhydrin. You will be provided with the materials and equipment listed

below.

Materials and equipment provided: 1. Unknown amino acid solution 15mL 12. glass rod 2 2. Glutamate (powder) 13. a squirt DI water bottle 3. 2% Ninhydrin reagent 15mL 14. 50mL volumetric flask 2 4. 0.2M acetate buffer (pH5.5)10mL 15. Pasteur pipette 2 5. medium test tubes 12 16. funnel x1 6. 1mL graduated pipette 1 17. 25mL graduated cylinder x1 7. 10mL graduated pipette 1 18. marker x1 B. 10mL volumetric pipette 1 19. Whatman #1 fitter paper 1 9. rubber bulb 1 20. a cuvette, spectrophotometer (shared) 10. 50 or 100mL beakers 3 21. Balances as provided in the lab 11. 250mL beaker 1 Materials and equipment provided: 1. Unknown amino acid solution 15mL 12. glass rod 2 2. Glutamate (powder) 13. a squirt DI water bottle 3. 2% Ninhydrin reagent 15mL 14. 50mL volumetric flask 2 4. 0.2M acetate buffer (pH5.5)10mL 15. Pasteur pipette 2 5. medium test tubes 12 16. funnel x1 6. 1mL graduated pipette 1 17. 25mL graduated cylinder x1 7. 10mL graduated pipette 1 18. marker x1 B. 10mL volumetric pipette 1 19. Whatman #1 fitter paper 1 9. rubber bulb 1 20. a cuvette, spectrophotometer (shared) 10. 50 or 100mL beakers 3 21. Balances as provided in the lab 11. 250mL beaker 1