You wish to express a particular peptide in E.Coli using high copy number plasmid. You have the amino acid sequence for the peptide. Explain the experimental process for generating and selecting the genetically engineered E.coli using restriction enzyme, ligases, E.coli, plasmid with neomycin resistance and the known amino acid sequences. What control element would you place on the plasmid to regulate expression? Design the reactor for the large-scale production of that particular peptide.