Question
Questions Q1 ) We need to study an enzyme activity that works best at pH equals to 4,Which one of the following you will choose?
Questions
Q1 ) We need to study an enzyme activity that works best at pH equals to 4,Which one of the following you will choose?
Buffer name | pka | Buffer capacity |
Acetic acid | 4.32 | 3.32-5.32 |
TRIS | 8.1 | 7.1-9.1 |
So we need to choose the buffer with the nearest pka to the desired pH
Dont forget that:
each buffer still maintains its buffer property in a pH around its pka 1
This is called the buffer capacity
The wk. acid/or wk base will be super in resisting changes in pH when their pka = the desired pH
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Q2) The pka of carbonic acid is 6.37. At which ranges of pH carbonic acid can work as a good buffer?
H2CO3 HCO3- + H+
Q3) The most effective buffer is obtained when [------] = [------] and thus pH = pka
Q4) When the pH of the solution is below the pka of the wk acid / or wk base, what is the predominant form of the buffer, is it the basic form or the acidic form? The protonated form or the deprotonated form?
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