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Recombinant antigen (vaccine) of Covid19 will be produced by the aerobic fermentation from bacteria of E. coli. 3M Biologics Sdn Bhd is interested in producing

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Recombinant antigen (vaccine) of Covid19 will be produced by the aerobic fermentation from bacteria of E. coli. 3M Biologics Sdn Bhd is interested in producing the protein commercially as it has high market value. As an Engineer, you are required to design a fermentor based on the following basis: Annual production: 130,000kg/ ear pure and dry product (antigen) Fermentation characteristics: Current maximum working volume, to date: 30L Maximum cell mass achieved (dry weight): 30g/L (dried weight) Production cell mass desired: 80g/L Maximum expression level of product: 0.05g/g cells (dried weight) Cell yield based on glucose consumed, Yx/s:0.5 Cell yield based on oxygen consumed, Yx/0:0.7 Sustainable specific growth rate, :0.3h1 (at 37C and pH 7.4) Lab fermentation time: 10h Broth rheology: Newtonian; maximum viscosity: 4cp Purification efficiency: 80% overall at laboratory scale Operating days: 330 actual operating days a year; 24 hours per day; 7 days per week Number of fermentor: 1 (one) From the information that has been given, determine: a) The volume and the dimension of the fermentor tank (working volume =70%, height to diameter ratio, ht/Dt=2.80) b) Size of the impeller (number of impeller =1, diameter of tank to diameter of impeller ratio, Dt/Di=3 ) c) Power number d) Oxygen transfer rate, OTR e) Heat transfer rate

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