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Results Table 1: Hill Reaction in NaCl Solutions (A600) Time (second) Change in Absorbance at 600nm Tube1 Blank Tube 2 Control Tube 3 1% NaCl

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Table 1: Hill Reaction in NaCl Solutions (A600)

Time (second)

Change in Absorbance at 600nm

Tube1 Blank

Tube 2 Control

Tube 3 1% NaCl Solution

Tube 4 2.5% NaCl Solution

Tube 5 5% NaCl Solution

Tube 6 10% NaCl Solution

0

-0.041

0.328

0.273

0.355

0.345

0.396

10

-0.113

0.246

0.171

0.225

0.228

0.271

20

-0.186

0.154

0.061

0.11

0.148

0.134

30

-0.25

0.095

-0.021

-0.006

0.067

0.092

40

-0.307

0.024

-0.095

-0.068

0.084

0.014

50

-0.366

-0.034

-0.153

-0.115

-0.053

-0.044

60

-0.408

-0.084

-0.214

-0.171

-0.086

-0.092

This is the table in our hill reaction lab. DCIP, spinach, buffer and salt is used. the results show blank should have been zero but all the values are negative. Tube 3 through 6 as NaCl concentration increased absorbance decreased. But is it not. Can you please write a conclusion and errors based on the data?

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