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Sequential frontal chromatography was carried out for a solute X. The input plot (what occurred at the column inlet) and the resulting sequential breakthrough plots

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Sequential frontal chromatography was carried out for a solute X. The input plot (what occurred at the column inlet) and the resulting sequential breakthrough plots at the column outlet are presented in the attached figure (note: the same time axis is used for both plots). Calculate the Langmuir isotherm parameters for this solute under these mobile phase conditions. The dimensions of the column are 20cm (length) 0.5cm (column diameter). The interstitial velocity inside the column is 17cm/min, interstitial porosity is 0.4 and intraparticular porosity is 0.55. What will be the maximum stationary phase concentration for this solute under these conditions

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