Serial dilutions of LDH from a 0.3 M lactate stock solution made in 0.1 M Tris buffer at pH 8 (this is the reaction buffer and the buffer in which NAD and LDH were prepared. 1) calculate the concentration of lactate in each of the 8 spectrophotometer tubes once you finish all 5 steps described (final volume 1 mL), (2) calculate the lactate concentration in each LDH reaction (final volume 3 mL), (3) determine the lactate concentration in each spectrophotometer tube if after you complete taking absorbance measurements (at equilibrium) you add 0.1 mL 0.3 M lactate (final volume 3.1 mL) and (4) determine the lactate concentration in each spectrophotometer tube if after you complete taking absorbance measurements (at equilibrium) you add 0.5 mL 0.3 M lactate (final volume 3.6 mL). Insert table below: 1. Label 8 spectrophotometer tubes (1.1 cm inner diameter) as shown to the right and add 1 ml buffer (0.1M Tris pH) to tubes 2 through 8. Leave tube #1 empty. 2. Add 1 ml 0.3 M lactate to tubes #1 and #2 as shown to the right. Cover tube #2 with parafilm and gently mix inversion. 3. Transfer 1 ml from tube #2 to tube #3. Cover tube #3 with parafilm and gently mix by inversion. 4. Continue to dilute the lactate solutions by (1) transferring 1 ml to the adjacent tube, (2) mixing that tube by inversion and (3) transferring 1 ml to the next tube. 5. Discard 1 ml from tube 38. Each tube will contain 1 ml lactate solution. Serial dilutions of LDH from a 0.3 M lactate stock solution made in 0.1 M Tris buffer at pH 8 (this is the reaction buffer and the buffer in which NAD and LDH were prepared. 1) calculate the concentration of lactate in each of the 8 spectrophotometer tubes once you finish all 5 steps described (final volume 1 mL), (2) calculate the lactate concentration in each LDH reaction (final volume 3 mL), (3) determine the lactate concentration in each spectrophotometer tube if after you complete taking absorbance measurements (at equilibrium) you add 0.1 mL 0.3 M lactate (final volume 3.1 mL) and (4) determine the lactate concentration in each spectrophotometer tube if after you complete taking absorbance measurements (at equilibrium) you add 0.5 mL 0.3 M lactate (final volume 3.6 mL). Insert table below: 1. Label 8 spectrophotometer tubes (1.1 cm inner diameter) as shown to the right and add 1 ml buffer (0.1M Tris pH) to tubes 2 through 8. Leave tube #1 empty. 2. Add 1 ml 0.3 M lactate to tubes #1 and #2 as shown to the right. Cover tube #2 with parafilm and gently mix inversion. 3. Transfer 1 ml from tube #2 to tube #3. Cover tube #3 with parafilm and gently mix by inversion. 4. Continue to dilute the lactate solutions by (1) transferring 1 ml to the adjacent tube, (2) mixing that tube by inversion and (3) transferring 1 ml to the next tube. 5. Discard 1 ml from tube 38. Each tube will contain 1 ml lactate solution