Show your calculations used to solve Questions $6-9$ below. NOTE: Molar mass for chemicals

related to this assignment can be found at the bottom of the page under "Useful Information."

$,5\mathrm{.}25M$ Tris

g $($you will calculate for a total volume of $380mL)$

Buffer A

$0\mathrm{.}5M$ Glycine

$25\%$ vlv Methanol

g

$d{H}_{2}O$ up to $3L$ final volume $($you will calculate for a total volume of $3$ litters$)$

Buffer B

$40\%(\frac{v}{v})$ Methanol

$mL$

$5\%(\frac{v}{v})$ Acetic Acid

$mL$

$d{H}_{2}O$ up to $0\mathrm{.}5L$ final volume $($you will calculate for a total volume of $0\mathrm{.}5$ liter$)$

$4\%SDS(\frac{m}{v})$

g $($you will calculate for a total volume of $550mL)$