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slallulidi y phase: negatively charged; Mobile phase: pH and salt concentration specific 1. Reverse phase chromatography Stationary phase: positively charged; Mobile phase: pH 2. and

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slallulidi y phase: negatively charged; Mobile phase: pH and salt concentration specific 1. Reverse phase chromatography Stationary phase: positively charged; Mobile phase: pH 2. and salt concentration Normal phase chromatography specific 3. Stationary phase: polar Hydrophilic-interaction ; Mobile phase: non-polar chromatography (HILIC) Stationary phase: polar 4. Affinity chromatography ; Mobile phase: polar 5. Anion exchange chromatography Stationary phase: Non- polar ; Mobile phase: polar 6. Cation exchange chromatography Stationary phase: has selective ligand; Mobile phase: pH and salt 1. Bias (\%error) Speed of analysis 2. \%RSD Portability 3. LOD or MDL Ruggedness 4. Slope "m" of the calibration curve 5. The time to analyze (or process) Accuracy one sample Detection limit 6. The number of samples that can be processed in a set amount of time (taking into consideration samples that can be done Sensitivity simultaneously) Throughput 7. Whether it is labor intensive Greenness 8. Whether it is durable and robust Detection limit be processed in a set amount of time (taking into consideration samples that can be done Sensitivity simultaneously) Throughput 7. Whether it is labor intensive 8. Whether it is durable and robust Greenness 9. Whether it can be used on-site or Cost in field and ease to transport 10. If it is environmentally friendly Precision 11. How expensive it is and how Ease of automation expansive is each run 12. Selectivity If it is capable of distinguishing the analyte from interferences slallulidi y phase: negatively charged; Mobile phase: pH and salt concentration specific 1. Reverse phase chromatography Stationary phase: positively charged; Mobile phase: pH 2. and salt concentration Normal phase chromatography specific 3. Stationary phase: polar Hydrophilic-interaction ; Mobile phase: non-polar chromatography (HILIC) Stationary phase: polar 4. Affinity chromatography ; Mobile phase: polar 5. Anion exchange chromatography Stationary phase: Non- polar ; Mobile phase: polar 6. Cation exchange chromatography Stationary phase: has selective ligand; Mobile phase: pH and salt 1. Bias (\%error) Speed of analysis 2. \%RSD Portability 3. LOD or MDL Ruggedness 4. Slope "m" of the calibration curve 5. The time to analyze (or process) Accuracy one sample Detection limit 6. The number of samples that can be processed in a set amount of time (taking into consideration samples that can be done Sensitivity simultaneously) Throughput 7. Whether it is labor intensive Greenness 8. Whether it is durable and robust Detection limit be processed in a set amount of time (taking into consideration samples that can be done Sensitivity simultaneously) Throughput 7. Whether it is labor intensive 8. Whether it is durable and robust Greenness 9. Whether it can be used on-site or Cost in field and ease to transport 10. If it is environmentally friendly Precision 11. How expensive it is and how Ease of automation expansive is each run 12. Selectivity If it is capable of distinguishing the analyte from interferences

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