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solution. You have 2.5ml of a 1mg/mL NaCl solution. You need to make 5 mL of a 0.25mg/mL HAVE (Stock solution) C 1mg/mL NaCl
solution. You have 2.5ml of a 1mg/mL NaCl solution. You need to make 5 mL of a 0.25mg/mL HAVE (Stock solution) C 1mg/mL NaCl V = ? mL Please note: NEED (New solution) C = 0.25mg/mL V = 5mL The needed/ new solution is more dilute (less concentrated) than the stock solution. It does not matter how much (volume) of stock solution one has, what matters is how much is needed to make a new solution of given concentration and volume. The units of volume and concentration on both sides of the equation must be the same. C*Vi C2 * V2 (1mg/mL) (V1mL) (0.25mg/mL) * (5mL) V1 mL (0.25mg/ml) * (5mL) = 1mg/mL 1.25 mg 1 mg/ml V = 1.25mL Even though you had 2.5mL of stock solution, only 1.25mL of it is needed to make the diluted solution. The final volume of the new solution is 5mL. You will need to add (V1-V2 = 5ml - 1.25mL) 3.75mL of diluent to the 1.25mL stock solution. Graduated cylinders (10mL, 100mL, 1000mL), serological pipettes (2mL, 5mL, 10ml, 25mL) and micropipettes are best used to make solutions accurately. **Explain and/or sketch out exactly how you would go about making the above solution given the above tools.
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