Question
What do you expect to see on your DNA gel if your colleague added the wrong restriction endonuclease to the strain Z sample, resulting in
What do you expect to see on your DNA gel if your colleague added the wrong restriction endonuclease to the strain Z sample, resulting in the plasmid being cut at only one site (and failing to release a fragment of interest)?
Strain Z: This E. coli strain contains a plasmid expressing the bacterial gene for CAT that confers resistance to chloramphenicol.
The strain Z sample would look like?
(a) (b) (c) (d) One band at the top, close to the well. One band far away from the well. at the bottom Two bands. No bands
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Microbiology A Systems Approach
Authors: Marjorie Kelly Cowan
4th edition
978-007340243, 73402435, 978-0073402437
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