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Substrate Treatment __ Sucrose ________ Treatment ___ NaCl/Sucrose __ Treatment __ EtOH __________ Treatment Treatment ___ EtOH/Sucrose __ __ Na saccharin ____ Time 10% 5%

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Substrate Treatment __Sucrose________ Treatment ___NaCl/Sucrose__ Treatment __EtOH__________ Treatment Treatment ___EtOH/Sucrose__
__Na saccharin____
Time 10% 5% 2.50% 10% 5% 2.50% 10% 5% 2.50% 10% 5% 2.50% 10% 5% 2.50%
(minutes)
0 0.995 0.978 0.755 1.172 1.137 1.095 1.065 1.018 1.018 1.078 1.053 1.050 0.926 0.974 1.010
30 0.959 1.001 0.970 1.010 1.007 1.073 1.124 1.038 1.050 1.032 1.058 1.047 0.928 0.961 1.039
60 0.856 0.909 0.915 1.073 1.041 1.037 1.040 1.018 0.878 1.017 0.991 1.038 0.947 0.971 1.080
90 0.909 0.847 0.887 1.028 1.091 1.132 0.976 0.961 0.881 1.027 1.026 1.037 0.936 0.993 1.071
120 0.953 0.933 0.985 1.035 1.056 1.087 1.018 1.081 1.102 0.868 0.855 0.853 0.838 0.899 0.995
150 0.834 0.897 0.949 1.059 1.080 1.110 0.964 1.130 1.107 0.997 1.031 1.015 0.957 1.016 1.162
180 1.010 0.941 0.969 1.029 1.107 1.119 0.677 1.130 1.122 0.970 1.011 1.028 0.987 1.061 1.249
210 0.958 0.996 1.015 1.060 1.069 1.226 0.762 1.157 1.185 0.994 1.041 1.052 1.021 1.099 1.280
240 1.009 1.065 0.972 1.069 1.080 1.211 0.735 1.171 1.187 0.985 1.032 1.028 1.002 1.146 1.309
270 1.050 1.009 1.186 1.040 1.082 1.169 0.761 1.261 1.144 0.976 1.068 1.094 1.057 1.183 1.351
300 1.098 1.056 1.230 1.058 1.074 1.174 0.838 1.219 1.135 0.981 1.085 1.132 1.089 1.215 1.393
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1) Calculate growth rates. This has been done for you - see dataset supplied on Moodle. 2) Create 5-line charts using Excel or Google Sheets (See Tutorials on Moodle). Plot data from table 3 (Growth vs. time). The three different concentrations for each treatment should be represented on a single graph. Make sure you create a legend, which identifies the three concentrations (10%, 5%, 2.5%). Questions - answer as a part of your assignment. 1) Explain the processes of glycolysis and fermentation. What are the main differences between these two processes? 2) Which concentration is best for each of your substrates? 3) What is the best substrate for growth of yeast? Justify your answer. 4) What is the worst substrate for growth of yeast? Justify your answer. 1) Calculate growth rates. This has been done for you - see dataset supplied on Moodle. 2) Create 5-line charts using Excel or Google Sheets (See Tutorials on Moodle). Plot data from table 3 (Growth vs. time). The three different concentrations for each treatment should be represented on a single graph. Make sure you create a legend, which identifies the three concentrations (10%, 5%, 2.5%). Questions - answer as a part of your assignment. 1) Explain the processes of glycolysis and fermentation. What are the main differences between these two processes? 2) Which concentration is best for each of your substrates? 3) What is the best substrate for growth of yeast? Justify your answer. 4) What is the worst substrate for growth of yeast? Justify your

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