Summary of Catalytic Strategies in the Full Reaction of Chymotrypsin and a Protein Substrate. Draw the arrows depicting the flow of electrons in this mechanism. Locate these different strategies: General base catalysis: (activating Ser to be a better Nu) 1 o General base catalysis: (activating H20 to be a better Nu) 2 o Stabilizing the tetrahedral transition state with hydrogen bonding 3 o Group transfer to create a better LG 4 O N. N. N IN-protein Ala HN oo o N 6 0 HN-protein CH o Sef 195 NN-H -Ser- Aspa HNN Aspan " HISST Hiss tetrahedral intermediate second prodcut leaves active site H,O enters active site Contact HAN ) N AlaHN- -protein 0 HO first prodcut leaves active site N Ala N N-protein NH 2nd tetrahedral intermediate 20 Ala H o new substrate enters active site NH Sor 196 H-O H H-O H 0 NN-H NH Ser 10 Hisz Aspice N HO o His Asp 102