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Sunset Yellow is a dye that absorbs strongly in the blue region of the visible spectrum, with a max of 470nm. Suppose a student carried
Sunset Yellow is a dye that absorbs strongly in the blue region of the visible spectrum, with a max of 470nm. Suppose a student carried out an experiment similar to the spectrophotometric determination of copper(II) using Sunset Yellow. The student used a 0.0400mM stock solution of the dye, prepared four standard solutions to measure the absorbance at max, and constructed a calibration curve. The student then measured the absorbance of a sample of Sunset Yellow with an unknown concentration at 470nm. Because of the strong absorption of the sample, 1.0mL of the solution was diluted to 10.0mL. The absorbance of the diluted sample of the unknown solution of Sunset Yellow was 0.500. Calculate the concentration of the unknown Sunset Yellow solution (before dilution). concentration mM Sunset Yellow is a dye that absorbs strongly in the blue region of the visible spectrum, with a max of 470nm. Suppose a student carried out an experiment similar to the spectrophotometric determination of copper(II) using Sunset Yellow. The student used a 0.0400mM stock solution of the dye, prepared four standard solutions to measure the absorbance at max, and constructed a calibration curve. The student then measured the absorbance of a sample of Sunset Yellow with an unknown concentration at 470nm. Because of the strong absorption of the sample, 1.0mL of the solution was diluted to 10.0mL. The absorbance of the diluted sample of the unknown solution of Sunset Yellow was 0.500. Calculate the concentration of the unknown Sunset Yellow solution (before dilution). concentration mM
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