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Sweetzyme Q is a commercial preparation of glucose isomerase immobilized in a porous spherical particle ( Novo Industries, Denmark ) . Plotted below are initial
Sweetzyme Q is a commercial preparation of glucose isomerase immobilized in a porous
spherical particle Novo Industries, Denmark Plotted below are initial rates of xylose
isomerization catalyzed by Sweetzyme Q in units of mols xylose isomerizedmg
particlemin The external concentration of xylose is M the density of the catalyst is
gcm
and the measured KM is M Assume that the maximum activity plotted in
the figure would not increase if the particle diameter were further reduced. ref Blanch
and Clark, Biochemical Engineering, p
i Estimate the effective diffusivity of xylose in the Sweetzyme Q catalyst. Use
definition of Actual Modulus.
ii What is the largest potential source of error in the calculation in part i
iii Using the effective diffusivity calculated in part i for a particle diameter of
m estimate the internal effectiveness factor.
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