Table 1: A summary of retention times, peak areas and peak heights obtained for the phenol standard solutions. Retention time Peak area Concentration 1x10SM Peak height 3 167 5.265 25 240 2x10-5 M 5.277 48 141 5 900 6x105M 5.238 158 049 19 448 8x105M 5.224 200 137 24 699 1x10-M 5.194 247 217 30 099 1x10M 5.309 2 526 113 305 349 Additional questions 1. For this experiment, you were provided with a stock solution of 1x10% M phenol and required to make a series of dilutions from the stock solution to prepare five standard solutions containing 1 x 104 M, 8 x 10-5M, 6 x 10-6 M, 2 x 105 M and 1 x 105 M phenol. Briefly explain how you would prepare each solution from the stock solution of 1x103M phenol in a 10 mL volumetric flask. 2. In Table 1, slight shifts in the retention time of phenol were observed for different solutions. To what factor(s) can the shifts in retention time be attributed? Why would this be a problem when using the retention time as a means of identification? How can this be improved? 3. What is the name of the quantification method used in this experiment? What advantages and disadvantages does this method have in comparison to other quantification methods that you know? 4. Comment on the relationship between peak areas/peak heights and the concentration of phenol in the different solutions (Table 1). Is it advisable to use the peak height to estimate the concentration of phenol in the TCP sample? Under what conditions could the peak height be anunreliable basis for quantification