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The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH7.0 and 20C to produce Glutamic acid for the

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The bacterium Corynebacterium glutamicum has been aerobically cultured in a batch fermenter vessel on glucose at pH7.0 and 20C to produce Glutamic acid for the manufacture of Mono Sodium Glutamate, a flavour compound for the food industry. The time course data for a typical batch culture of this bacterium in a fermenter vessel are given below: \begin{tabular}{|c|c|c|c|} \hline Time(h) & Cellconcentration(g1) & Glucoseconcentration(g1) & Glutamicacidconcentration(g1) \\ \hline 0 & 1.222 & 100 & 0 \\ \hline 5 & 1.492 & 98.0 & 2.5 \\ \hline 10 & 1.649 & 97.0 & 7.5 \\ \hline 15 & 2.719 & 90.4 & 20.0 \\ \hline 20 & 4.482 & 76.9 & 34.0 \\ \hline 45 & 7.390 & 48.1 & 43.0 \\ \hline 30 & 12.183 & 20.6 & 47.5 \\ \hline 40 & 21.250 & 1.1 & 48.3 \\ \hline & 20.086 & 3.4 & 48.0 \\ \hline \end{tabular} Based on the above data, determine the maximum specific growth rate of Corynebacterium glutamicum. \begin{tabular}{l} 0.1min1 \\ 0.063h1 \\ 0.0034min1 \\ 1s1 \\ 0.08g1s1 \\ \hline 0.003h1 \\ 0.1h1 \\ 0.74h1 \\ \hline 21.2s1 \end{tabular} none of the above The fermenter vessel is to be harvested for the glutamic acid in the liquid phase. once the carbon substrate (glucose) is completely consumed. How many production scale fermenters, each of 100 m3 operating liquid volume, would be needed to make 20,000 tonnes per year of Glutamic acid? Each batch vessel is operated for the harvest time of complete carbon substrate utilisation (45 hours). and takes an additional 3 hours to load with liquid medium and inoculate with bacterial culture, and 2 hours to drain and sterilise after the aerobic batch fermentation. Take a process year to be 320 days. (Write in the box below the number of fermenter vessels needed. Do not enter any text, only a number)

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