The catalytic reaction rate by the enzyme according to the concentration of the substrate is shown in the table.

It showed the reaction rate when the inhibitor was not present and the inhibitor concentration was 10 MM. The initial concentration of the enzyme is 1 MM.

Answer the following question using the data from the table (indicate the answer to the second decimal place with the unit)

(1) Calculate the maximum reaction speed, Vmax and Michaelis constant, and Km.

(2) Find the reaction rate constant of the reaction stage in which ES complex is converted to product.

(3) What kind of inhibitor is an inhibitor?

(4) Find V'm and KI in the presence of inhibitors.

(Sl. WM 40 60 90 120 200 300 400 Reaction rate, M/min No inhibitor Inhibitor 4.44 2.11 5.45 2.86 6.43 3.75 7.06 4.44 8.00 5.71 8.57 6.67 8.89 7.27