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The default setting in the Nanodrop Instrument subtracts the absorbance at 340 nm from the absorbance at 280 nm. Explain (in two sentences or less)

The default setting in the Nanodrop Instrument subtracts the absorbance at 340 nm from the absorbance at 280 nm. Explain (in two sentences or less) why this is not necessarily sufficient to correct for background in determining the concentration of your protein.

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