The enzyme with a K=2.7x10^-3M was inhibited with a competitive inhibitor (K_i=3.1x10^-5 M). when add inhibitor concentration 1.2*10^-6 M The reaction speed was reduced by 25% if the reaction was to return to the same speed at the concentration of The initial substrate is 3.6x10^-4M. Calculate the concentration of the substrate to be added

3. Km=2.7103M (KI=3.1105M) 1.2106M 25% 3.6104M (15 ) The enzyme with a K2.710M was inhibited with a competitive inhibitor (K3.110M). The reaction was reduced by 25% if the reaction was to return to the same speed at the concentration of The initial substrate is 3.610M. Calculate the concentration of the substrate to be added