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The figure shows the separation of two enantiomers on a chiral stationary phase. Separation of enantiomers of Ritalin by HPLC with a chiral stationary phase.
The figure shows the separation of two enantiomers on a chiral stationary phase. Separation of enantiomers of Ritalin by HPLC with a chiral stationary phase. [Data from R. Bakhtiar, L. Ramos, and F. L. S. Tse, "Quantification of Methylphenidate in Plasma Using Chiral Liquid-Chromatography/Tandem Mass Spectrometry: Application to Toxicokinetic Studies," Anal. Chim. Acta 2002, 469, 261.] (a) From tr and w1/2, find N for each peak. (b) From tr and w1/2, find the resolution. (c) Given tm=1.62min, use Equation 23-33 with the average N to predict the resolution
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