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The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1. 95C for 2 minutes 2. 95C

The following is the PCR reaction that is programmed in the thermocycler to amplify the cheek cell DNA: 1. 95C for 2 minutes 2. 95C for 30 seconds 3. 58.1C for 30 seconds 4. 72C for 45 seconds 5. Repeat steps 2-4 thirty (30) times 6. 72C for 3 minutes 7. Hold at 16C indefinitely Which steps represent the denaturation, annealing and extension steps? 7 a. Step 2 = Denature; Step 4 = Anneal; Step 6 = Extend b. Step 4 = Denature; Step 6 = Anneal; Step 7 = Extend c. Step 2 = Denature; Step 3 = Anneal; Step 4 = Extend d. Step 3 = Denature; Step 4 = Anneal; Step 6 = Extend

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