The glucose-oxidase enzyme reaction is used in the assay for glucose concentration. In the laboratory experiment, the conditions of the assay are adjusted such that reaction rate is proportional to glucose concentration. Assume the reaction follows the Michaelis Menten rate equation, shown in Equation 1. Then, describe the steps taken in the assay procedure to obtain a linear correlation between reaction rate and glucose concentration in the assay.

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Where rp= reaction rate, mMol/(Lmin) [Eo] = Enzyme concentration, mMol/L [S] = Substrate concentration, mMol/L Km = Michaelis Menten constant, mMol/L The Km for the glucose oxidase reaction is ~10 mM (or mMol/L)