The growth rate of E. coli in synthetic medium can be expressed by Monod kinetics as 0.935S g/l-hr 0.71+S where S is the concentration
The growth rate of E. coli in synthetic medium can be expressed by Monod kinetics as 0.935S g/l-hr 0.71+S where S is the concentration of a limiting substrate, glucose. You are going to cultivate E. coli in a steady-state CSTR (working volume: 10 1)with a flow rate of 7 1/hr. The initial substrate concentration is 10 g/l and the apparent cell yield (Yx/s) is 0.6. The feed stream is sterile. a. What will be the doubling time of the cells in the CSTR? b. What will be the cell and substrate concentrations in the outlet stream?
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