The inactivation of a microorganism by high hydrostatic pressures at 35 C of 100- 600 MPa showed the following parameters: Z of 588 MPa and deltaV of -10 cm3/mol. If at 100MPa a D value of 0.7531 min-1 was obtained. Generate the inactivation curve of the microorganism at 400 MPa to reduce the microbial population by 5 cycles. If the CUT was 1 min at 400 MPa and during this time the population decreased 0.8 cycles, generate again the inactivation curve considering the effect of the CUT.