The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: [S] (mol/L) 5 10 20 50 100 200 V[(mol/L) min] 22 39 65 102 120 135 (a) Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting clear answers? (b) Now use a Lineweaver-Burk plot to analyze the same data. Does this work better? (c) Finally, try an Eadie-Hofstee plot of the same data. (d) If the total enzyme concentration was 1 nmol/L, how many molecules of substrate can a molecule of enzyme process in each minute? (e) Calculate kcat/KM for the enzyme reaction. Is this a fairly efficient enzyme