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An unknown bacterium is tested for tryptophanase activity. The media used to test the organism was prepared according to the following recipe: To 1000
An unknown bacterium is tested for tryptophanase activity. The media used to test the organism was prepared according to the following recipe: To 1000 mL distilled water add: 5 g Lysine 1 g Glucose 5 g Acid Hydrolyzed Peptone 3 g Yeast Extract 0.02 g Brom Cresol Purple The inoculated media is incubated for 48 hours at 37C. Following incubation, the media is briefly mixed by vortexing and Kovac Reagent added. The resulting assay for tryptophanase activity is shown below: Intepret the results: O The bacterium is negative for tryptophanase activity. The bacterium is positive for tryptophanase activity. O The result cannot be determined. O the bacterium did not grow in the media. Submit Answer Tries 0/99
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