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The pH values 7.7 and 8.5 are both 0.4 pH units away from the pKa of Tris, but in opposite directions. In a sentence or

The pH values 7.7 and 8.5 are both 0.4 pH units away from the pKa of Tris, but in opposite directions. In a sentence or two, relate this fact to moles of acid and base determined for #1 above.

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