Effects of H2O2 on viability of SHSY5Y neuronal cells pretreated with HRP determined by XTT.

(a) 96-well plates were seeded with 1 x 105 cells/well and incubated overnight in the absence or presence of HRP (0.1 mg/ml) at 37ºC.

(b) After washing with serum free medium, cells were exposed to doubling dilutions of the H2O2.stock (1 mM) for 1 h.

(c) XTT was added and the cells incubated overnight at 37ºC.

(d) Absorbance of plates was read at 450 nm in a multi well plate spectrophotometer.

(e) Absorbance data from XTT assay is presented on my beckett for analysis