The recommended concentration of Kanamycin in an LB medium is 50 g/mL. Typically we make 1000 times more concentrated (known as 1000X) stock solution, which

The recommended concentration of Kanamycin in an LB medium is 50 μg/mL. Typically we make 1000 times more concentrated (known as 1000X) stock solution, which is 50 mg/mL. The stock solution is then aliquoted and kept at -20 °C. Suppose you want to make 25 mL of 1000X solution; calculate the amount of Kanamycin. Assume that you are growing 200 mL of E. coli, calculate the volume of the stock solution of Kanamycin to the culture so that the final concentration becomes 50 μg/mL.