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The value of KM and rmax for an enzyme are 0.004 kmol/m3 and 10 kmol/m3s. It was immobilized to the surface of acrylamide-based polymers that
The value of KM and rmax for an enzyme are 0.004 kmol/m3 and 10 kmol/m3s. It was immobilized to the surface of acrylamide-based polymers that can be assumed to have spherical shape (diameter = 1 mm). The effectiveness of the immobilized enzyme was found to be 70% of the free enzyme when the concentration of the substrate was 0.5 kmol/m3. The reaction was carried out in a stirred reactor with an agitation speed of 50 rpm. a. (5 pts) Estimate the concentration of the substrate at the surface of the immobilized enzyme. b. (5 pts) Estimate .
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