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there are 20 sites from which study samples (Water samples from the sea) were collected and 5 species of aquatic vertebrates with which this research

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there are 20 sites from which study samples (Water samples from the sea) were collected and 5 species of aquatic vertebrates with which this research is interested, then: In terms of statistics, which analysis should be used to determine if the locations differ in the presence of the 5 species? and could you please explain the mechanics of this analysis in detail with an example? Thank you

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