This is a graph of absorbance vs relative concentration of 4-cholesten-3-one, the product of the reaction catalyzed by cholesterol oxidase. The lowest concentration matches the lowest absorbance, and larger concentrations can be matched, one-to-one, to the higher absorbance spectra.

a) Plot the absorbance at 240 nm vs the concentration of 4-cholesten-3-one (assume that the given relative concentrations are in M). This can be done with a graphing program. Hint: The plot should be linear.

b) From the slope of the plot in (a), calculate the molar extinction coefficient of 4-cholesten-3-one at 240 nm. The cuvette width = 1.0 cm.

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