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Eastern Sand Darters (a remarkable fish species in the Great Lakes watershed) have multiple populations in both Ontario and Ohio. Ontario populations are expected to be small, isolated and partially inbred because of habitat fragmentation whereas Ohio populations have large continuous habitat so little isolation or inbreeding is expected. Does habitat fragmentation affect the amount of inbreeding in different populations? a. Fish samples from Ontario populations had an F value of 0.10, as did the fish samples from Ohio populations. What do these F values tell us about these two populations and how do they relate to the amount of habitat fragmentation in the two areas? (2) b. You also calculated mean Fst values for both Ontario populations and Ohio populations. You found that Ontario populations have a mean FST = 0.12 and Ohio populations have a mean Fst = 0.07. What do these values tell us about these two populations? (1) c. Since FST = (HT - Hs)/Ht where HT = 2p'q', explain how p' is calculated? (1) Eastern Sand Darters (a remarkable fish species in the Great Lakes watershed) have multiple populations in both Ontario and Ohio. Ontario populations are expected to be small, isolated and partially inbred because of habitat fragmentation whereas Ohio populations have large continuous habitat so little isolation or inbreeding is expected. Does habitat fragmentation affect the amount of inbreeding in different populations? a. Fish samples from Ontario populations had an F value of 0.10, as did the fish samples from Ohio populations. What do these F values tell us about these two populations and how do they relate to the amount of habitat fragmentation in the two areas? (2) b. You also calculated mean Fst values for both Ontario populations and Ohio populations. You found that Ontario populations have a mean FST = 0.12 and Ohio populations have a mean Fst = 0.07. What do these values tell us about these two populations? (1) c. Since FST = (HT - Hs)/Ht where HT = 2p'q', explain how p' is calculated? (1)