To prepare 2fold Gel- neutralization solutions we added 1)100 ml 10 fold concentration Ham's F-12 2)400 ml water 3)2.438 g Sodium hydrogen carbonate Then stirring until dissolved 4)16.1 ml 3M HEPES solution. 5)0 g Glucose To prepare 2fold gel-neutralization solutions we added 1)175.5 ml 10 fold concentration Ham's F-12 2)324.5 ml water 3)4.28g Sodium hydrogen carbonate 4) 28.3 ml 3M HEPES solution Then stirring until dissolved 4)16.1 ml 3M HEPES solution. 5)0 g Glucose For 5fold: 1)75 ml 10 fold concentration Ham's F-12 2)75 ml water 3)1.829g Sodium hydrogen carbonate 4) 10ml 3M HEPES solution 5)3.564 g D-glucose

The questions are: 1)What are the concentrations of all components 2)what happens of we don't use Ham's f-12 solutions and we add instead of his volume to water 3)what is effect of all components on Collagen? 4)What are the differences between 2fold, 2fold and 5 fold