Transcriptional stop sequence intron +1 TATA box * G TAA AUG UAA Here is a diagram of a human gene (genel) identifying locations 1-8 by the numbered stars. a. Where in this gene would a single base pair insertion cause the mRNA to become one base pair longer, but would NOT be able to cause a frameshift mutation (disrupted translation due to shifting out of frame)? Indicate the location(s) using the numbered stars. (1 mark) b. Where in this gene would a single base pair insertion cause an error in splicing? (1 mark) Researchers have two different cultures of human fibroblast cells (strains A and B) which have both lost the function of gene1. Results from a semi-quantitative RT-PCR and a western blot are shown below. WT Strain A Strain B WT Strain A Strain B b) Propose a possible location in genel where a point mutation could explain the results seen for strain A. Indicate the possible gene region/s using the figure of the gene and the numbered stars. Justify in the light of both experiments. (2 marks) c) Propose a possible location in genel where a point mutation could explain the result seen in strain B? Indicate the possible gene region/s using the figure of the gene and the numbered stars. Justify in the light of both experiments. (2 marks)