Tyrosine and tryptophan absorb light in the UV region of the spectrum. Suppose that a

protein of molecular weight $26,000\frac{g}{m}ol$ contains two tryptophane amino acids and six

tyrosine amino acids. The absorption is recorded from a cell of path length $1cm,$ holding

the protein at a concentration of $1m\frac{g}{m}l.$

a$)$ Calculate the absorbance and the percent transmission of the protein at $280nm,$ using

the extinction coefficients given below:

b$)$ What would the measured absorbance be at $288nm$ for the protein solution?Tyrosine and tryptophan absorb light in the UV region of the spectrum. Suppose that a

protein of molecular weight $26,000$ g$/$mol contains two tryptophane amino acids and six

tyrosine amino acids. The absorption is recorded from a cell of path length $1$ cm$,$ holding

the protein at a concentration of $1$ mg$/$ml$.$

a$)$ Calculate the absorbance and the percent transmission of the protein at $280$ nm$,$ using

the extinction coefficients given below:

$\backslash$epsi $280\backslash$epsi $288$

Trp $5690$ cm$-1$ M $-14815$ cm$-1$ M $-1$

Tyr $1280$ cm$-1$ M $-1385$ cm$-1$ M $-1$

b$)$ What would the measured absorbance be at $288$ nm for the protein solution?