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Use Beers Law to calculate the actual BSA concentration of the 1.0 mg/ml solution, based on the OD280 measurements you made on the U2800 or
Use Beers Law to calculate the actual BSA concentration of the 1.0 mg/ml solution, based on the OD280 measurements you made on the U2800 or U2900 instruments. How well does your calculated BSA concentration (based on your spectrophotometric measurement) agree with the expected concentration of 1 mg/ml? Speculate on the reason for any differences.
C = A/ * l
A = 0.617
= 0.66 cm-1 (mg/ml)-1
L = 1 cm
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