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USE THE FOLLOWING SEQUENCE TO CREATE PRIMERS. PLEASE FOLLOW THE DIRECTIONS. MAKE SURE YOU INCLUDE REVERSE PRIMER. THANK YOU FOR YOUR HELP! 1 gtgcatcagc gtacacgtcg
USE THE FOLLOWING SEQUENCE TO CREATE PRIMERS. PLEASE FOLLOW THE DIRECTIONS.
MAKE SURE YOU INCLUDE REVERSE PRIMER.
THANK YOU FOR YOUR HELP!
1 gtgcatcagc gtacacgtcg gccaggccgg agttcagatc ggtaatgcct gctgggagct 61 ctactgettg gaacatggaa ttcagcctga tggacatatg ccgtcagaca agaccgttgg 121 aagcggcgat gactccttca acacgttttt ctctgagact ggagctggga agcacgttcc 181 ccgtgctgtc tttgttgatc ttgagcccac tgtcgtcgac gaagttagga ctggaactta 241 cagacagctc ttccaccccg aacaactcat cactggtaag gaagatgctg ccaacaacta 301 cgcccgaggt cactacacga tcggcaagga gatcgtagac gtggtgctgg ataggatccg 361 caagctgtct gttcagtgta ccggactcca gggctttttg attttccact ccttcggcgg 421 cggcactggt tctggattta cctcccttct tatggaacga ctttcggttg actatggcaa 481 gaaatccaag ctcgaattcg ccgtctaccc tgctcctcag gtctctaccg ctgttgttga 541 accctacaac tccatcctca ctacgcacac taccctcgag cactccgact gcgcattcat 601 ggtcgacaat gaggctattt atgacatctg ccgccgtaac ctggatatcg agaggccgac 661 ctacaccaac cttaacaggc tgattggtca gatcgtttcc tcaataacag cctctcttcg 721 gttcgatgga gcccttaatg tcgacctcac ggaattccag acgaacttgg tcccataccc 781 cagaatccac ttccccctcg taacctacgc ccctgtcatc toggctgaga aagcctacca 841 tgaacagcta tctgtcggtg agatcaccaa tgettgette gagcccgcta accagatggt 901 aaaatgcgac ccacgtcacg caaatacat gettgctgc atgttgtaca ggggtgatot 961 tgtacctaag gacgtcaacg ccgccatcgc caccatcaag actaagagga ccatccagtt 1021 cgtcgactgg tgceocactg gtttcaaagt tggcatcaac taccagcccc ctaccgtcgt 1081 cccaggaggt gacetogo Use the sequence posted on Primers must : Amplify a 300 bp amplicon (+/- 50 bp) Be -50% GC content (+/- 5%) Start or end with a G or C Be 18-20 bp long each Be correct sequence (write out sequence of primers only-not whole amplicon) Be highlighted on what you turn in (where they are on the sequence)
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